Researchers compared their new scattering coefficient (μs) method with the traditional optical density (OD) method for clotting blood.
The Optical Density method measures the concentration of substances in the plasma by quantifying how much light it absorbs.
They used Beer–Lambert law.
μs is the scattering coefficient
P0 = transmitted power through buffer (no plasma scatterers)
P(t) transmitted optical power through the plasma
d thickness of glass
When a graph of Scattering coefficient vs time is drawn, a temporal timing shift occurs compared to Optical Density measurements. It shows a function of plasma concentration.
As blood plasma changes from a liquid to a gel (clot), the scattering coefficient μₛ increases steadily means the structure inside the plasma (fibrin fibers and networks) becomes more complex and scatters light more strongly.
Here μₛ method provides extra information such as specific times (t50%, t90%), time difference (Δt), and the clot formation rate (CFR), and structure of clot formation.
Source: https://iopscience.iop.org/article/10.1088/2057-1976/ae103c
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